Related Experiment Video

Updated: Apr 17, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

16.7K

PinX1 inhibits cell proliferation, migration and invasion in glioma cells

Peng-Jin Mei1, Yan-Su Chen, Ying Du

  • 1Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221002, Jiangsu Province, China.

Medical Oncology (Northwood, London, England)
|February 21, 2015
PubMed

Insights

PinX1 suppresses glioma cell proliferation and metastasis by regulating cell cycle arrest and inhibiting telomerase reverse transcriptase (hTERT). This suggests PinX1 acts as a tumor suppressor gene in glioma progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • PinX1 (Telomerase Pin2/TERF1-interacting protein) is known to induce apoptosis and inhibit proliferation in various cancer cells.
  • Decreased PinX1 expression correlates with poor prognosis and increased metastasis in several cancers.
  • The specific role of PinX1 in gliomas remains largely uninvestigated.

Purpose of the Study:

  • To elucidate the function of PinX1 in glioma cell proliferation, cell cycle regulation, and invasion.
  • To investigate the molecular mechanisms underlying PinX1's effects in glioma cells.

Main Methods:

  • Cell proliferation assays were performed to assess the impact of PinX1 on glioma cell growth.
  • Cell cycle analysis was conducted to determine the phase-specific arrest induced by PinX1.
  • Western blotting was used to evaluate the expression levels of key proteins, including cell cycle regulators and telomerase components.
  • Migration and invasion assays, along with the assessment of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) expression, were employed.

Main Results:

  • PinX1 significantly reduced glioma cell proliferation by arresting cells in the G1 phase of the cell cycle.
  • PinX1 suppressed the expression of human telomerase reverse transcriptase (hTERT), a core component of telomerase.
  • PinX1 inhibited glioma cell wound healing, migration, and invasion.
  • These effects were associated with the suppression of MMP-2 and the upregulation of TIMP-2 expression.

Conclusions:

  • PinX1 demonstrates potent anti-proliferative and anti-metastatic effects in gliomas.
  • PinX1 functions by regulating cell cycle progression and inhibiting hTERT expression.
  • PinX1 may serve as a potential tumor suppressor gene, offering a therapeutic target for glioma treatment.

More Related Videos

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

14.1K
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs08:35

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Published on: July 20, 2017

13.8K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.3K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
5.0K