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Somatostatin concentration and binding in the rat hypothalamus and striatum during severe insulin-induced
M N Rodriguez-Sanchez1, E Arilla
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Madrid, Spain.
Hypoglycemia was induced by administration of insulin (40 I.U./kg) to 24 h fasted rats. Somatostatin-like immunoreactivity (SLI) and 125I-Tyr11-somatostatin binding were measured in the striatum and hypothalamus at the onset of hypoglycemic coma (5-10 min). No significant changes in SLI concentration were detected in either site although the total number of specific somatostatin receptors in the striatum membranes, but not in the hypothalamus, decreased in insulin-injected rats when compared with the control group.
Hypoglycemia was induced by administration of insulin (40 I.U./kg) to 24 h fasted rats. Somatostatin-like immunoreactivity (SLI) and 125I-Tyr11-somatostatin binding were measured in the striatum and hypothalamus at the onset of hypoglycemic coma (5-10 min). No significant changes in SLI concentration were detected in either site although the total number of specific somatostatin receptors in the striatum membranes, but not in the hypothalamus, decreased in insulin-injected rats when compared with the control group.