Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genome-wide variation in and between two closely related underutilised horsegram species (<i>Macrotyloma axillare</i> and <i>M. uniflorum</i>, Fabaceae).

AoB PLANTS·2026

Same author

Genetic variation and synonymous cultivars in the USDA lychee (<i>Litchi chinensis</i> Sonn.) collection assessed using genome-wide SNPs.

Genetic resources and crop evolution·2025

Same author

Novel breeding resources for the underutilised legume, lablab, based on a pangenome approach.

Breeding science·2025

Same author

Agricultural landscape genomics to increase crop resilience.

Plant communications·2025

Same author

Drought Response in the Transcriptome and Ionome of Wild and Domesticated <i>Lablab purpureus</i> L. Sweet, an Underutilized Legume.

Plant-environment interactions (Hoboken, N.J.)·2025

Same author

'Domesticability': were some species predisposed for domestication?

Trends in ecology & evolution·2025

Same journal

Leveraging target enrichment and genome skimming (Hyb-Seq) of herbarium collections to unlock timber DNA barcoding.

Applications in plant sciences·2026

Same journal

Detecting cryptic ghost lineage introgression in four-taxon genomic datasets.

Applications in plant sciences·2026

Same journal

HapAsmbl: A reference-aided pipeline for assembling haplotypes in Nanopore amplicon sequence data of polymorphic populations.

Applications in plant sciences·2026

Same journal

HybSuite: An integrated pipeline for hybrid capture phylogenomics from reads to trees.

Applications in plant sciences·2026

Same journal

Detecting introgression from phylogenetic invariant site patterns using machine learning.

Applications in plant sciences·2026

Same journal

tanggle: An R package for the visualization of phylogenetic networks.

Applications in plant sciences·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Transcriptome sequencing and marker development for four underutilized legumes.

Mark A Chapman¹

¹Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom ; Centre for Underutilised Crops, University of Southampton, Southampton SO17 1BJ, United Kingdom.

Applications in Plant Sciences

|February 21, 2015

Summary

This summary is machine-generated.

This study sequenced and analyzed four underutilized legume crops, identifying genetic markers. This research supports breeding efforts for climate-resilient crops to enhance food security.

Keywords:

beans drought legumes microsatellites simple sequence repeats (SSRs)transcriptome

More Related Videos

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Published on: June 9, 2015

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Related Experiment Videos

Last Updated: Apr 17, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Published on: June 9, 2015

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Area of Science:

Agricultural Science
Genomics
Plant Breeding

Background:

Climate change and population growth threaten global food and nutrition security.
Underutilized crops from extreme environments may possess valuable adaptive traits.

Purpose of the Study:

To characterize the genetic resources of four underutilized legume crops.
To identify genetic markers for comparative genomics and phylogenetic analysis.

Main Methods:

Transcriptome sequencing, assembly, and annotation of four legume species.
Identification of microsatellite markers and conserved orthologous markers.
Ground-truthing of markers on diverse legume germplasm.

Main Results:

Successfully sequenced, assembled, and annotated transcriptomes for four underutilized legumes.
Identified species-specific microsatellite markers and a conserved orthologous marker set.
Validated marker utility across a range of legume varieties.

Conclusions:

Provides foundational genomic data for underutilized legume species.
Enables comparative mapping and phylogenetic studies within the legume family.
Facilitates the investigation of genetic variation and adaptive traits for crop improvement.