Plasma membrane lipids modulate the response to water deprivation in rat kidney

The lipid composition of biological membranes determines many of the cellular responses to stimuli such as hormones and drugs. It is known that the neonate has differences in renal function compared to the adult individual, which determine the characteristics of pharmacokinetics and pharmacodynamics at this age. We studied the lipid composition of renal plasma membranes, the renal response to water deprivation, and the activity of plasma membrane gamma-glutamyl transpeptidase (GGTP) in newborn, 21-d-old, and adult rat. It was found that the cholesterol/phospholipid (CH/PL) ratio of newborn membranes was significantly higher than that of membranes from 21-d-old and adult rats, which were similar. These findings paralleled low ability to concentrate urine in the newborn and higher sensitivity of the neonatal kidney to water deprivation. Kidney membranes from 21-d-old and adult rats showed similar lipid composition, and the response to water deprivation was also similar. The changes observed in GGTP activity at different ages varied with the cholesterol content of kidney plasma membranes. These findings suggest participation of the lipid composition of kidney membranes in modulation of the renal response to water deprivation.