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The Mos proto-oncogene maps near the centromere on mouse chromosome 4
F Propst1, G F Vande Woude, N A Jenkins
1NCI-Frederick Cancer Research Facility, BRI-Basic Research Program, Maryland 21701.
Abstract:
The Mos proto-oncogene, the cellular homolog of the transforming gene of Moloney murine sarcoma virus, was originally assigned to mouse chromosome 4 using independent panels of mouse/hamster somatic cell hybrids. By in situ hybridization to metaphase chromosomes and standard genetic backcrosses, we have confirmed this assignment and determined that Mos maps near the centromere in a region devoid of other markers. We have also identified a restriction fragment length polymorphism (RFLP) that defines two alleles of the Mos locus in selected inbred strains of laboratory mice. Using the RFLP, we determined the strain distribution pattern for the Mos gene in three sets of recombinant inbred strains and in five strains congenic for histocompatibility antigen genes localized on chromosome 4. These results establish Mos as a useful marker in a poorly characterized region of the mouse genome. In addition, these results will facilitate the genetic analysis of the Mos locus.
Insights
Researchers mapped the Mos proto-oncogene to mouse chromosome 4, near the centromere. This finding establishes Mos as a valuable genetic marker for further analysis of this understudied genome region.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- The Mos proto-oncogene is the cellular homolog of the Moloney murine sarcoma virus transforming gene.
- Previous studies assigned the Mos gene to mouse chromosome 4 using somatic cell hybrids.
Purpose of the Study:
- To confirm the chromosomal assignment of the Mos gene.
- To refine the localization of Mos on mouse chromosome 4.
- To identify and utilize a restriction fragment length polymorphism (RFLP) for Mos to facilitate genetic analysis.
Main Methods:
- In situ hybridization to metaphase chromosomes.
- Standard genetic backcrosses.
- Restriction fragment length polymorphism (RFLP) analysis.
- Strain distribution pattern analysis in recombinant inbred and congenic strains.
Main Results:
- The Mos gene was confirmed to be located on mouse chromosome 4.
- Mos was mapped to a region near the centromere, an area with few other known markers.
- A restriction fragment length polymorphism (RFLP) for the Mos locus was identified, revealing two alleles in laboratory mice.
- The strain distribution pattern of the Mos RFLP was determined across various mouse strains.
Conclusions:
- The Mos gene's precise mapping and the identification of its RFLP establish it as a valuable genetic marker.
- This research facilitates further genetic analysis of the Mos locus and the poorly characterized region of the mouse genome where it resides.