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Campylobacter species identification based on polymorphism of DNA encoding rRNA
P Moureau1, I Derclaye, D Gregoire
1Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
Abstract:
Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsaliensis, displayed a characteristic pattern. Although some bands may be common to different species, the simplicity of the hybridization pattern enabled us to discriminate among the different species of Campylobacter.
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