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Layer-by-layer assembled cell instructive nanocoatings containing platelet lysate.

Sara M Oliveira1, Vítor E Santo1, Manuela E Gomes1

  • 13B's Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, University of Minho, AvePark, Caldas das Taipas, Guimarães 4806-909, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães 4806-909, Portugal.

Biomaterials
|February 22, 2015
PubMed
Summary

Researchers developed novel nanocoatings using sulfated polysaccharides and platelet lysate (PL) to control cell behavior. These biomimetic materials effectively deliver growth factors, promoting stem cell adhesion and proliferation for advanced tissue engineering applications.

Keywords:
Cell behaviorGrowth factorsInstructive surfacesLayer-by-layer assemblingPlatelet derivativePlatelet lysate

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Controlled delivery of growth factors (GFs) is crucial for regulating cell behavior in tissue engineering constructs.
  • Platelet lysate (PL) is a cost-effective source of multiple GFs, but its incorporation into constructs requires strategic methods.
  • The presentation, stability, and conformation of GFs significantly influence their biological instructions.

Purpose of the Study:

  • To develop strategies for controlled incorporation of platelet lysate (PL) into nanocoatings.
  • To evaluate the capability of sulfated polysaccharides in incorporating PL and its associated growth factors (VEGF, FGFb, PDGF).
  • To assess the impact of these biomimetic multilayers on human adipose-derived stem cells (hASCs).

Main Methods:

  • Layer-by-layer assembly was used to incorporate PL into nanocoatings with polysaccharides of varying sulfation degrees (SD) and charges.
  • Heparin and marine polysaccharides were utilized to test PL and GF adsorption.
  • Short-term cultures of high passage human adipose-derived stem cells (hASCs) were used to evaluate cellular responses.

Main Results:

  • The nature of the polysaccharide and its sulfation degree (SD) critically influenced the adsorption of PL and key growth factors (VEGF, FGFb, PDGF).
  • Sulfated polysaccharide-PL multilayers demonstrated efficient promotion of morphological changes in hASCs.
  • These nanocoatings enhanced serum-free adhesion and proliferation of high passage hASCs (P > 5).

Conclusions:

  • Sulfated polysaccharides are effective carriers for platelet lysate (PL) incorporation into nanocoatings.
  • The developed biomimetic multilayers offer a versatile platform for creating instructive devices with tunable PL delivery.
  • This approach holds promise for advancing the fabrication of sophisticated tissue engineering scaffolds and cell-instructive devices.