Related Experiment Video
Updated: Apr 17, 2026

10:22
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
Published on: December 4, 2015
9.4K
Fractionation of Plasmodium-infected human red blood cells to study protein trafficking
Simone Külzer1, Verena Bittl, Jude M Przyborski
1Research School of Biology, The Australian National University, Canberra, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|February 23, 2015
Abstract:
Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.

