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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
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Moving from discovery to validation in circulating microRNA research.
Paolo Verderio1, Stefano Bottelli, Chiara M Ciniselli
11 Unit of Medical Statistics, Biometry and Bioinformatics, National Cancer Institute, IRCCS Foundation, Milan - Italy.
The International Journal of Biological Markers
|February 24, 2015
Summary
Reproducibility between miRNA identification and validation cards is not fully satisfactory. A technical validation step is needed after discovery and before independent series validation for accurate cancer research.
Area of Science:
- Molecular biology
- Genomics
- Cancer research
Background:
- MicroRNAs (miRNAs) are key regulators in cancer development.
- Quantitative real-time polymerase chain reaction (qPCR) is standard for miRNA expression analysis.
- Low-density arrays are used for miRNA identification and validation.
Purpose of the Study:
- To assess the reproducibility between high-throughput and customized qPCR arrays for miRNA analysis.
- To evaluate the technical robustness of miRNA validation methods in colorectal cancer screening.
Main Methods:
- Analysis of plasma-circulating miRNAs in 60 colorectal cancer screening participants.
- Comparison of miRNA expression data obtained from two types of qPCR arrays.
Main Results:
- Reproducibility between the two qPCR array types was not fully satisfactory.
- A concordance correlation coefficient of 0.69 was observed, indicating moderate agreement.
Conclusions:
- A technical validation step is crucial after high-throughput miRNA discovery.
- This validation should precede the independent series validation phase.
- Ensuring technical robustness is essential for reliable miRNA research in cancer.
