Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

102.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
102.1K
Sanger Sequencing01:57

Sanger Sequencing

780.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
780.8K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

868
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
868
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

689
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
689
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.8K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.8K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.3K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating relationship inference from low-quality DNA using a conditional simulation framework.

Forensic science international. Genetics·2026
Same author

Advancing forensic SNP typing: Insights from an interlaboratory study of the FORCE panel.

Forensic science international. Genetics·2026
Same author

Preparing for Shotgun Sequencing in Forensic Genetics-A Workflow for Analysis of Monozygotic Twins.

Genes·2026
Same author

The battle of the titans: Comparative performance of Kintelligence, microarray, and shotgun sequencing in ten Estonian cases with unidentified human remains.

Forensic science international. Genetics·2026
Same author

Preparing for shotgun sequencing in forensic genetics - Benchmarking of tools for read mapping, genotype calling, and imputation.

Forensic science international. Genetics·2026
Same author

Age prediction of contributors to two-person DNA mixtures via deconvolution of autosomal DNA methylation profiles.

Forensic science international. Genetics·2026

Related Experiment Video

Updated: Apr 17, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.8K

Next generation sequencing and its applications in forensic genetics.

Claus Børsting1, Niels Morling1

  • 1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.

Forensic Science International. Genetics
|February 24, 2015
PubMed
Summary

Next-Generation Sequencing (NGS) offers advanced forensic genetic analysis by examining multiple markers simultaneously. This technology uncovers new genetic variations, improving evidence interpretation and statistical weight in forensic casework.

Keywords:
Forensic geneticsNext generation sequencingReviewSingle-molecule sequencing

More Related Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.9K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

35.1K

Related Experiment Videos

Last Updated: Apr 17, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.8K
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

16.9K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

35.1K

Area of Science:

  • Genetics
  • Forensic Science
  • Molecular Biology

Background:

  • Next-Generation Sequencing (NGS) has revolutionized genetic research over the past decade.
  • NGS technologies have matured, achieving high quality suitable for clinical diagnostics.
  • Forensic genetics is increasingly exploring NGS for casework applications.

Purpose of the Study:

  • To review the advancements in NGS and single-molecule sequencing.
  • To discuss the potential applications of NGS in forensic genetics.
  • To highlight how NGS can enhance forensic genetic analysis.

Main Methods:

  • Review of scientific literature and conference presentations on NGS in forensics.
  • Discussion of NGS capabilities for analyzing multiple genetic markers (STRs, SNPs, indels, mRNA) concurrently.
  • Exploration of single-molecule sequencing principles.

Main Results:

  • NGS enables simultaneous analysis of diverse genetic markers, surpassing standard methods.
  • Discovery of novel Short Tandem Repeat (STR) alleles and deeper understanding of STR locus variation.
  • Detailed sequence information from NGS aids in interpreting complex mixtures and strengthens evidence statistics.

Conclusions:

  • NGS presents significant opportunities to advance forensic genetic casework.
  • The technology offers enhanced data generation for individual identification and relationship testing.
  • Further integration of NGS is expected to improve the accuracy and power of forensic genetic evidence.