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Related Experiment Videos

Monkey pox in humans: current results.

S S Marennikova1, E M Shelukhina, G R Matsevich

  • 1Moscow Research Institute of Viral Preparations, U.S.S.R. Ministry of Health.

Acta Virologica
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

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Researchers recommend improved methods for rapid detection of orthopoxvirus, including passive haemagglutination (PHA) and ELISA, for efficient field diagnosis of monkeypox. Serologic diagnosis using ELISA-adsorption (ELISA-A) is valuable for epidemiology and detecting asymptomatic cases.

Area of Science:

  • Virology
  • Immunology
  • Epidemiology

Background:

  • Monkeypox is a zoonotic viral disease caused by the orthopoxvirus.
  • Accurate and rapid laboratory diagnosis is crucial for effective disease control and epidemiological surveillance.
  • Existing diagnostic methods may have limitations in field settings.

Purpose of the Study:

  • To evaluate and recommend improved methods for the rapid detection of orthopoxvirus antigen.
  • To assess the utility of ELISA-adsorption (ELISA-A) for serologic diagnosis of monkeypox.
  • To determine the application range and limitations of ELISA-A for epidemiological studies and detecting inapparent infections.

Main Methods:

  • Passive haemagglutination (PHA) using dried stable red blood cells.
  • Enzyme-linked immunosorbent assay (ELISA) for antigen detection.

Related Experiment Videos

  • ELISA-adsorption (ELISA-A) for serologic diagnosis.

    • Main Results:

    • Passive haemagglutination (PHA) and ELISA offer improved, rapid detection of orthopoxvirus antigen.
    • ELISA-adsorption (ELISA-A) is valuable for serologic diagnosis, epidemiological studies, and detecting inapparent monkeypox infections.
    • The application range and limitations of ELISA-A were determined.

    Conclusions:

    • Improved methods like PHA and ELISA enhance rapid laboratory diagnosis of orthopoxvirus under field conditions.
    • ELISA-adsorption (ELISA-A) is a valuable tool for monkeypox seroepidemiology and identifying asymptomatic cases.
    • Further understanding of ELISA-A's scope and limitations supports its application in public health initiatives.