Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glycosaminoglycans01:23

Glycosaminoglycans

8.0K
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
8.0K
Proteoglycans01:05

Proteoglycans

5.3K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
5.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.2K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.2K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.9K
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

7.9K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The enteroviral protease target LSM14A operates outside of P-bodies to augment antiviral innate immunity.

bioRxiv : the preprint server for biology·2026
Same author

Mass spectrometry analysis of young and aged mice and human Alzheimer's disease with Lewy body pathology using on-slide tissue digestion.

Analytical and bioanalytical chemistry·2026
Same author

Spatial Profiling of Glycosaminoglycans (GAGomics) From Laser Microdissected Mouse Brain.

Molecular & cellular proteomics : MCP·2026
Same author

Optimization of LC-ExD methods for glycopeptides on an Omnitrap-Orbitrap platform.

Analytical and bioanalytical chemistry·2026
Same author

Updates to the Minimum Information Required for A Glycomics Experiment (MIRAGE) guidelines.

Analytical and bioanalytical chemistry·2026
Same author

Update and New Implementation of the MIRAGE Reporting Guidelines for Mass Spectrometry Experiments in Glycoscience.

Molecular & cellular proteomics : MCP·2025

Related Experiment Video

Updated: Apr 17, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

17.2K

Analysis of Glycosaminoglycans Using Mass Spectrometry.

Gregory O Staples1, Joseph Zaia1

  • 1Center for Biomedical Mass Spectrometry, Dept. of Biochemistry, Boston University School of Medicine.

Current Proteomics
|February 24, 2015
PubMed
Summary

Mass spectrometry (MS) provides advanced methods for analyzing glycosaminoglycans (GAGs). This review highlights MS-based techniques for determining GAG structure and function, crucial for biomedical research.

Keywords:
Mass spectrometrychondroitin sulfatedermatan sulfateglycosaminoglycanheparan sulfateheparinhyaluronankeratan sulfateproteoglycan

More Related Videos

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.4K
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

13.3K

Related Experiment Videos

Last Updated: Apr 17, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

17.2K
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.4K
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

13.3K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Glycobiology

Background:

  • Glycosaminoglycans (GAGs) are vital polysaccharides on cell surfaces and in extracellular matrices.
  • GAG structure, including sulfation and uronic acid epimerization, dictates protein binding and biological activity.
  • Accurate and rapid analysis methods are essential for understanding GAG functions.

Purpose of the Study:

  • To review mass spectrometry (MS)-based approaches for GAG analysis.
  • To detail methods for determining GAG sulfation, acetylation, and uronic acid epimerization.
  • To cover tissue extraction and chromatographic techniques compatible with MS.

Main Methods:

  • Utilizing modern ionization techniques for accurate molecular weight determination of GAG oligosaccharides.
  • Employing liquid chromatography-mass spectrometry (LC/MS) for online GAG analysis.
  • Developing new tandem mass spectrometry (MS/MS) methods for detailed GAG structure elucidation.

Main Results:

  • MS enables precise determination of GAG molecular weights and mixture distributions.
  • LC/MS interfaces allow for real-time analysis of GAGs.
  • Emerging tandem MS methods offer enhanced fine structure analysis of GAGs.

Conclusions:

  • Mass spectrometry is a powerful tool for comprehensive GAG analysis in biomedical research.
  • MS-based methods facilitate the study of GAG structure-function relationships.
  • This review provides a summary of current and emerging MS techniques for GAG analysis.