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Updated: Jul 24, 2026
10:22
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells
A Giordano1, P Whyte, E Harlow
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|September 8, 1989
Summary
The cellular protein p60 interacts with adenovirus E1A and cdc2 kinase. This p60-cdc2 complex exhibits cell cycle-dependent histone H1 kinase activity, primarily during interphase, suggesting distinct cell cycle regulatory roles.
Area of Science:
- Cellular biology
- Virology
- Molecular biology
Background:
- p60 is a cellular protein that interacts with the adenovirus E1A protein.
- p60 forms a complex with cdc2 protein kinase in both infected and uninfected cells.
Purpose of the Study:
- To investigate the function of the p60-cdc2 complex.
- To determine the cell cycle-specific activity of the p60-cdc2 complex.
Main Methods:
- Immunoprecipitation of p60 and cdc2.
- Assay of histone H1 kinase activity in immune complexes.
Main Results:
- The p60-cdc2 complex exhibits histone H1 kinase activity.
- This activity is cell cycle-dependent, peaking during interphase.
- A previously identified cdc2-p62/cyclin complex shows maximal activity during mitotic metaphase.
Conclusions:
- The p60-cdc2 complex plays a distinct role in cell cycle regulation during interphase.
- Different cdc2-containing complexes may have specialized functions in controlling cell cycle progression.
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