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Updated: Apr 17, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
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[A multienzyme bioreactor based on a chitinase complex].

E G Vlakh, E A Ponomareva, T B Tennikova

    Prikladnaia Biokhimiia I Mikrobiologiia
    |February 25, 2015
    PubMed
    Summary

    Researchers developed a novel heterogeneous biocatalyst using chitinolytic enzymes from Clostridium paraputrificum immobilized on a polymer matrix. This biocatalyst

    Area of Science:

    • Biocatalysis and enzyme immobilization
    • Microbial enzyme technology
    • Polymer science in biotechnology

    Background:

    • Chitinolytic enzymes are crucial for chitin degradation.
    • Immobilization of enzymes enhances stability and reusability.
    • Clostridium paraputrificum is a source of valuable microbial enzymes.

    Purpose of the Study:

    • To develop a heterogeneous biocatalyst for chitinolytic enzymes.
    • To immobilize enzymes from Clostridium paraputrificum onto a macroporous polymer matrix.
    • To investigate the activity of immobilized endochitinase and N-acetylglucosaminidase.

    Main Methods:

    • Isolation of chitinolytic enzyme complex from Clostridium paraputrificum culture medium.
    • Multistep immobilization of enzymes onto a macroporous monolithic minidisc using a macromolecular spacer.

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  • Assay of endochitinase and N-acetylglucosaminidase activity in the heterogeneous biocatalyst.

    • Main Results:

    • Successful preparation of a heterogeneous biocatalyst with immobilized chitinolytic enzymes.
    • Demonstration of endochitinase and N-acetylglucosaminidase activity in the immobilized enzyme complex.
    • The immobilization method utilizing a macromolecular spacer was effective.

    Conclusions:

    • A stable and active heterogeneous biocatalyst for chitinolytic enzymes was successfully developed.
    • The immobilized enzyme complex retains significant endochitinase and N-acetylglucosaminidase activity.
    • This biocatalyst holds potential for applications in chitin degradation and related biotechnologies.