Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors01:30

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors

2.9K
Angiotensin-converting enzyme (ACE), a vital component of the renin-angiotensin-aldosterone system, is abundant in lung endothelial cells. ACE converts the inactive decapeptide, angiotensin I, into the active octapeptide, angiotensin II. This potent vasoconstrictor narrows blood vessels, increasing resistance to blood flow and elevating blood pressure. Angiotensin II also stimulates aldosterone production, encouraging kidney cells to reabsorb more sodium and water from urine, thereby increasing...
2.9K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

4.3K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.3K
Antihypertensive Drugs: Direct Renin Inhibitors01:25

Antihypertensive Drugs: Direct Renin Inhibitors

1.9K
The renin-angiotensin-aldosterone system (RAAS) is an intricate physiological pathway involving numerous enzymes and hormones, including renin, angiotensin-converting enzyme (ACE), angiotensin I and II, and aldosterone. Imbalances within this system increase the production of angiotensin II and aldosterone. Increased angiotensin II levels promote vasoconstriction and blood pressure elevation. Concurrently, higher aldosterone levels stimulate sodium and water reabsorption in the kidneys,...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IL-2 and the thymus/weight index are inversely correlated with gestational age: a sign of Th1/Th2 imbalance in preterm infants and a possible connection with atopic dermatitis.

European annals of allergy and clinical immunology·2025
Same author

Urocortin 2 - a protective effect in hypertension?

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2023
Same author

Comprehensive geriatric assessment in primary care practices: a multi-centered, cross-sectional study in Krakow, Poland.

Hippokratia·2022
Same author

Protumorogenic Potential of Pancreatic Adenocarcinoma-Derived Extracellular Vesicles.

Folia biologica·2020
Same author

Arterial structure and function and its short- and long-term changes after bariatric surgery.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2020
Same author

Influence of Sociodemographic, Behavioral and Other Health-Related Factors on Healthy Ageing Based on Three Operative Definitions.

The journal of nutrition, health & aging·2019

Related Experiment Video

Updated: Apr 17, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

59.6K

Human monocyte subsets exhibit divergent angiotensin I-converting activity.

M Rutkowska-Zapała1, M Suski2, R Szatanek1

  • 1Department of Clinical Immunology, Polish-American Institute of Pediatrics.

Clinical and Experimental Immunology
|February 25, 2015
PubMed
Summary
This summary is machine-generated.

Human monocytes, particularly classical monocytes, express key enzymes in the renin-angiotensin-aldosterone system (RAAS). This suggests monocytes play a role in regulating blood pressure and vascular tone through RAAS mechanisms.

Keywords:
angiotensinangiotensin-converting enzymesmonocyte subpopulationsrenin-angiotensin-aldosterone system

More Related Videos

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

7.7K
On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

11.5K

Related Experiment Videos

Last Updated: Apr 17, 2026

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

59.6K
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

7.7K
On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

11.5K

Area of Science:

  • Immunology
  • Cardiovascular Physiology
  • Molecular Biology

Background:

  • The renin-angiotensin-aldosterone system (RAAS) is crucial for regulating vascular tone and blood pressure.
  • Immune cells, specifically monocytes (MO), are increasingly recognized for their potential role within the RAAS.

Purpose of the Study:

  • To investigate the expression and activity of angiotensin-converting enzyme type 1 (ACE1) and ACE2 in human monocytes and their subsets.
  • To elucidate the role of monocyte subsets in RAAS-mediated vascular homeostasis.

Main Methods:

  • Analysis of ACE1 and ACE2 mRNA and protein expression in human monocyte subsets (classical and non-classical).
  • In vitro assessment of angiotensin II (Ang II), Ang-(1-9), and Ang-(1-7) generation by monocytes.
  • Comparison of enzyme activity across different monocyte populations and peripheral blood mononuclear cells (PBMC).

Main Results:

  • Classical monocytes (CD14(++)CD16(-)) exhibited the highest ACE1 and ACE2 mRNA expression, with ACE2 levels nearly double ACE1.
  • ACE1 and ACE2 protein expression was most prominent in monocytes and classical monocytes within PBMC.
  • Classical monocytes showed the highest Ang II generation, while non-classical monocytes (CD14(+)CD16(++)) produced significantly more vasoprotective Ang-(1-7) than Ang II.

Conclusions:

  • Human monocytes, especially classical and non-classical subsets, are involved in RAAS-related mechanisms that may regulate vascular homeostasis.
  • Non-classical monocytes display a vasoprotective phenotype, potentially through enhanced Ang-(1-7) formation, and are associated with the vascular endothelium.