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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

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Related Experiment Video

Updated: Apr 17, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

Nanoparticle-based caspase sensors.

Dusica Maysinger¹, Eliza Hutter

¹Department of Pharmacology & Therapeutics, McGill University, 3655 Promenade Sir-William-Osler, Room 1314, McIntyre Medical Sciences Building, Montreal, QC H3G 1Y6, Canada.

Nanomedicine (London, England)

|February 25, 2015

Summary

This summary is machine-generated.

Nanotechnology offers novel nanoparticle-based assays for precise measurement of caspase enzyme activity, crucial for understanding cell death and inflammation. These advanced methods overcome limitations of traditional techniques, enabling sensitive in vitro and in vivo analysis.

Keywords:

biosensors cancer cells caspase activity fluorescence gold nanoparticles microglia neurons quantum dots

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Last Updated: Apr 17, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

Area of Science:

Biotechnology
Nanotechnology
Biochemistry

Background:

Enzymatic activities are key indicators of physiological and pathological processes.
Caspases, cysteine-dependent aspartate-directed proteases, are critical in cell death and inflammation.
Accurate measurement of caspase activity requires specific, precise, and sensitive assays.

Purpose of the Study:

To review conventional methods for measuring caspase activity and their limitations.
To explore the advantages and limitations of emerging nanoparticle-based assays for caspase activity detection.
To provide an overview of nanotechnology applications in enzymatic activity sensing.

Main Methods:

Discussion of conventional caspase activity assays.
Overview of nanoparticle-based assay strategies.
Analysis of in vitro and in vivo sensing capabilities.

Main Results:

Conventional methods for caspase activity measurement have inherent limitations.
Nanoparticle-based assays present emerging strategies for caspase sensing.
These novel assays offer potential for enhanced specificity, precision, and sensitivity.

Conclusions:

Nanotechnology provides advanced tools for enzymatic activity measurement.
Nanoparticle-based assays are a promising frontier for studying caspases.
Further development is needed to fully leverage these techniques for biological research and diagnostics.