Related Experiment Video
Updated: Apr 17, 2026
Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
Published on: June 20, 2025
flg2 as a potential biomarker of acute cerebral ischemic-reperfusion injury
Ping Zhang1, Fei Cao2, Nian Xiong2
1Department of Geriatrics and Neurology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Abstract:
Cerebral ischemic-reperfusion injury as an inflammatory and microcirculation dysfunction pathological condition negatively affects the clinical outcome of stroke patients. The novel inflammatory procoagulant protein fgl2 has been reported to play a role in some inflammatory and coagulation dysregulation diseases. This study aimed to examine the relationship between fgl2 expression and infarct size in an acute cerebral ischemic-reperfusion rat model. We studied fgl2 mRNA and protein expressions in cerebral tissue and peripheral macrophages, and the expressions of several inflammatory factors (TNF-α, IL-1β, MCP-1, and IL-8) in serum samples from rats with acute cerebral ischemic-reperfusion injury. Fiber microthrombosis in situ contributed to the microvascular thrombosis in acute cerebral ischemic-reperfusion injury, and fgl2 expression tended to strongly correlate with cerebral infarct size. The expression levels of the other inflammatory factors significantly increased but weakly correlated with cerebral infarct size. These findings support the potential of fgl2 level as a novel biomarker of acute cerebral ischemic-reperfusion injury.
Insights
Fibrinogen-like protein 2 (fgl2) expression strongly correlates with cerebral infarct size in stroke injury models. This finding suggests fgl2 may serve as a novel biomarker for acute cerebral ischemic-reperfusion injury.
Area of Science:
- Neuroscience
- Immunology
- Pathology
Background:
- Cerebral ischemic-reperfusion injury is a complex condition involving inflammation and microcirculation dysfunction, impacting stroke patient outcomes.
- Fibrinogen-like protein 2 (fgl2), a novel inflammatory procoagulant protein, is implicated in inflammatory and coagulation disorders.
Purpose of the Study:
- To investigate the relationship between fgl2 expression and infarct size in an acute cerebral ischemic-reperfusion rat model.
- To evaluate fgl2's potential as a biomarker for acute cerebral ischemic-reperfusion injury.
Main Methods:
- Assessed fgl2 mRNA and protein expression in rat cerebral tissue and peripheral macrophages.
- Measured serum levels of inflammatory factors (TNF-α, IL-1β, MCP-1, IL-8).
- Correlated fgl2 expression with cerebral infarct size and other inflammatory markers.
Main Results:
- Fiber microthrombosis was observed, contributing to microvascular thrombosis in the injury model.
- fgl2 expression demonstrated a strong correlation with cerebral infarct size.
- Other inflammatory factors increased significantly but showed weak correlation with infarct size.
Conclusions:
- fgl2 expression is closely linked to infarct size in acute cerebral ischemic-reperfusion injury.
- fgl2 shows potential as a novel biomarker for assessing the severity of acute cerebral ischemic-reperfusion injury.
