Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Carbon-dioxide Fixation

Carbon-dioxide Fixation

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Enzymes

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cascade System Bridging Xanthine Oxidation and L-Arginine for Highly Effective and Non-Toxic Surface Microbial Decontamination.

Biotechnology and bioengineering·2025

Same author

Boosting Current Density of Electrocatalytic CO<sub>2</sub> Reduction using Metal-Enzyme Hybrid Cathodes.

Angewandte Chemie (International ed. in English)·2025

Same author

Conductive single enzyme nanocomposites prepared by in-situ growth of nanoscale polyaniline for high performance enzymatic bioelectrode.

Biosensors & bioelectronics·2024

Same author

Magnetic hollow fibers of covalent organic frameworks (COF) for pollutant degradation and adsorptive removal.

Environmental research·2024

Same author

Gas-phase degradation of VOCs using supported bacteria biofilms.

Biotechnology and bioengineering·2023

Same author

Activation of crosslinked lipases in mesoporous silica via lid opening for recyclable biodiesel production.

International journal of biological macromolecules·2022

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Published on: September 29, 2023

Cascade enzymatic reactions for efficient carbon sequestration.

Shunxiang Xia¹, Xueyan Zhao¹, Benjamin Frigo-Vaz¹

¹Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA; Biotechnology Institute, University of Minnesota, St Paul, MN 55108, USA.

Bioresource Technology

|February 25, 2015

Summary

This study explores reversible cascade enzyme reactions for energy-efficient carbon sequestration. These biocatalytic reactions show potential for intensified carbon capture, approaching the capacity of chemical absorbents.

Keywords:

Aconitase Biocatalysis Carbon capture and storage Carbon dioxide Isocitrate dehydrogenase

More Related Videos

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Apr 17, 2026

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture

Published on: September 29, 2023

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

Biocatalysis
Enzyme engineering
Carbon capture and storage (CCS)

Background:

Thermochemical processes for CCS have high capacity but low energy efficiency.
Enzyme-catalyzed reactions offer a potential alternative for energy-efficient carbon sequestration.

Purpose of the Study:

To investigate reversible cascade enzyme reactions for energy-efficient carbon sequestration.
To demonstrate intensified carbon capture using integrated enzymatic reactions.

Main Methods:

Integration of isocitrate dehydrogenase and aconitase from the RTCA cycle.
Utilizing pH control under ambient conditions to enhance thermodynamic driving force.
Quantifying carbon capture capacity of the biocatalytic system.

Main Results:

Cascade enzymatic reactions demonstrated intensified carbon capture.
Enhanced thermodynamic driving force for carbon conversion achieved via pH control.
Potential to capture 0.5 mol of carbon per mole of substrate at pH 6.

Conclusions:

Reversible cascade enzyme reactions offer an energy-efficient pathway for carbon sequestration.
Biocatalytic carbon capture can be intensified to levels comparable to chemical absorbents like MEA.
Enzyme engineering presents a promising strategy for sustainable carbon capture technologies.