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Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Updated: Apr 17, 2026

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

Published on: May 6, 2019

Biomedical studies by TOF-SIMS imaging.

Claudia Bich¹, David Touboul¹, Alain Brunelle¹

¹Institut de Chimie des Substances Naturelles, UPR2301, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

|February 25, 2015

Summary

This summary is machine-generated.

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides high-resolution molecular imaging for biomedical applications. This technique enables detailed mapping of organic compounds directly on tissue sections without complex sample preparation.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Area of Science:

Biomedical imaging
Surface analysis
Mass spectrometry

Background:

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) has seen increased applications over the last decade.
Advancements in cluster ion sources have improved TOF-SIMS capabilities.
The technique offers micrometer lateral resolution for molecular imaging.

Purpose of the Study:

To review the biomedical applications of TOF-SIMS imaging.
To highlight the suitability of TOF-SIMS for mapping organic compounds in biological tissues.
To showcase developments in TOF-SIMS for analyzing drugs and lipids in tissue sections.

Main Methods:

Utilizing TOF-SIMS with cluster ion sources for high-resolution imaging.
Analyzing molecular ions directly on tissue sections.
Minimal sample preparation required for TOF-SIMS analysis.

Main Results:

TOF-SIMS successfully maps organic compounds like drugs and lipids on animal and human tissue sections.
The method achieves lateral resolution at the micrometer level or below.
Demonstrated suitability for direct analysis of biological samples.

Conclusions:

TOF-SIMS is a powerful tool for biomedical imaging of organic molecules.
Its ability to analyze tissues directly with high resolution opens new avenues in biomedical research.
The technique is well-suited for applications in drug distribution and lipidomics studies.