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Updated: Apr 17, 2026

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Published on: April 6, 2022
Effects of iriflophenone 3-C-β-glucoside on fasting blood glucose level and glucose uptake
Ratree Pranakhon1, Chantana Aromdee2, Patchareewan Pannangpetch3
1Faculty of Natural Resources, Rajamangala University of Technology ISAN Sakon Nakhon Campus, Muang, Thailand.
Background:
One of the biological activities of agar wood (Aquilaria sinensis Lour., Thymelaeaceae), is anti-hyperglycemic activity. The methanolic extract (ME) was proven to possess the fasting blood glucose activity in rat and glucose uptake transportation by rat adipocytes.
Objective:
To determine the decreasing fasting blood glucose level of constituents affordable for in vivo test. If the test was positive, the mechanism which is positive to the ME, glucose transportation, will be performed.
Materials And Methods:
The ME was separated by column chromatography and identified by spectroscopic methods. Mice was used as an animal model (in vivo), and rat adipocytes were used for the glucose transportation activity (in vitro).
Result:
Iriflophenone 3-C-β-glucoside (IPG) was the main constituent, 3.17%, and tested for the activities. Insulin and the ME were used as positive controls. The ME, IPG and insulin lowered blood glucose levels by 40.3, 46.4 and 41.5%, respectively, and enhanced glucose uptake by 152, 153, and 183%, respectively.
Conclusion:
These findings suggest that IPG is active in lowering fasting blood glucose with potency comparable to that of insulin.
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