Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Optimal Harvesting Time for Selaginella doederleinii Hieron at a Fixed Location Using HPLC Fingerprinting and In Vitro Antiproliferation Assays.

Chemistry & biodiversity·2026
Same author

Quantitative Analysis of Plasmid DNA Distribution and Transgene Expression Following Cell Squeezing.

Annals of biomedical engineering·2026
Same author

Integrated isothermal shift assay and multi-omics identify melittin as a novel EGFR-targeting peptide to suppress NSCLC.

Journal of translational medicine·2026
Same author

Thecoperitoneal shunt placement for extensive spinal adhesive arachnoiditis or lumbosacral outlet obstruction with syringomyelia.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Is Rituximab Suitable for Patients with Idiopathic Membranous Nephropathy Who are Seronegative for Anti-PLA2R Antibody?

Drug design, development and therapy·2026
Same author

A scoring model based on oral contrast-enhanced ultrasound for the diagnosis of gastric cancer: A preliminary study.

Ultrasound (Leeds, England)·2026

Related Experiment Video

Updated: Apr 17, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.5K

Mechanical analysis of rat trabecular meshwork.

Jianyong Huang1, Lucinda J Camras, Fan Yuan

  • 1Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Durham, NC 27708, USA. fyuan@duke.edu.

Soft Matter
|February 25, 2015
PubMed
Summary

A new atomic force microscopy (AFM) technique measures trabecular meshwork (TM) stiffness in rat eyes. This method enables studying TM stiffness in smaller animal models, crucial for understanding glaucoma and outflow resistance.

More Related Videos

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

10.0K
Mechanical Control of Relaxation Using Intact Cardiac Trabeculae
07:51

Mechanical Control of Relaxation Using Intact Cardiac Trabeculae

Published on: February 17, 2023

1.6K

Related Experiment Videos

Last Updated: Apr 17, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye
09:03

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

10.5K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

10.0K
Mechanical Control of Relaxation Using Intact Cardiac Trabeculae
07:51

Mechanical Control of Relaxation Using Intact Cardiac Trabeculae

Published on: February 17, 2023

1.6K

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Biomechanics

Background:

  • Trabecular meshwork (TM) stiffness is vital for regulating intraocular pressure and outflow resistance in healthy and glaucomatous eyes.
  • Current methods for measuring TM stiffness are limited to larger animal or human donor eyes, posing challenges for small animal models like rats.
  • Investigating TM stiffness in rat models is crucial for understanding glaucoma pathogenesis and developing new treatments.

Purpose of the Study:

  • To develop and validate a novel atomic force microscopy (AFM) technique for measuring the stiffness of trabecular meshwork (TM) tissue in rat eyes.
  • To establish a reliable method for quantifying TM stiffness in small animal models, facilitating research into glaucoma and ocular fluid dynamics.

Main Methods:

  • Rat eyes were enucleated, perfused with Evans blue tracer, and the anterior segment dissected.
  • Atomic force microscopy (AFM) with a gold-coated colloid tip was used to indent corneal, TM, and uveoscleral tissues.
  • Indentation data were analyzed using a novel mathematical model, assuming neo-Hookean material properties, to calculate apparent initial Young's moduli (E0)(app).

Main Results:

  • The study successfully determined the apparent initial Young's moduli (E0)(app) for rat TM and cornea.
  • TM stiffness was found to be significantly lower (162 Pa) than corneal stiffness (6189 Pa) (p < 0.01).
  • The developed AFM technique provides quantitative measurements of TM stiffness in rat eyes.

Conclusions:

  • A new AFM-based technique enables accurate measurement of trabecular meshwork (TM) stiffness in rat eyes.
  • This methodology opens avenues for using rat models to investigate TM biomechanics and its role in ocular diseases like glaucoma.
  • Future research can leverage this technique to explore age-related changes, effects of glaucoma, and therapeutic interventions on TM stiffness.