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Updated: Apr 17, 2026

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Published on: March 16, 2017
[The interaction between SGLT1 or GLUT2 glucose transporter and the cytoskeleton in the enterocyte as well as Caco2
Abstract:
The distribution of cytoskeleton elements (microtubules and actin filaments) and SGLT1 or GLUT2 glucose transporter in enterocyte of rat intestine and Caco2 cell during hexose absorption has been considered. The alteration of SGLT1 and GLUT2 transporter distribution in absorptive cell of intestine villus depending on maltose concentration has been determined using the confocal microscope. The colocalization of the transporters and actin has been revealed. The increase of vesicles number close to microtubules in the apical part of cell during absorption of high hexoze concentration has been found by electron microscope. The fact together with the transporter and actin as well as actin and α-tubulin colocalizations can prove the participation of cytoskeleton elements in glucose transporter movement to apical membrane of the cells studied.
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Glucose Absorption Into the Small Intestine
Glucose Transporters
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Secondary Active Transport
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