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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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KdmA, a histone H3 demethylase with bipartite function, differentially regulates primary and secondary metabolism in

Agnieszka Gacek-Matthews1, Luke M Noble2, Clemens Gruber3

  • 1Fungal Genetics and Genomics Unit, Department of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Life Sciences, Campus Tulln, Tulln, 3430, Austria.

Molecular Microbiology
|February 26, 2015
PubMed

Insights

Aspergillus nidulans KdmA, a histone demethylase, regulates gene expression and stress responses. Its absence impacts primary and secondary metabolism, affecting cell viability under specific conditions.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Fungal Biology

Background:

  • Histone demethylases regulate gene expression and chromosome structure.
  • The KDM4 family, including Aspergillus nidulans KdmA, targets H3K9 and H3K36 methylation.
  • Histone H3 trimethylation at K9 (H3K9me3) and K36 (H3K36me3) are key epigenetic marks.

Purpose of the Study:

  • To characterize the function of Aspergillus nidulans KdmA, a KDM4 family histone demethylase.
  • To investigate KdmA's role in transcriptional regulation and its impact on gene expression.
  • To determine KdmA's influence on fungal stress responses and secondary metabolite biosynthesis.

Main Methods:

  • Mass spectrometry for histone modification analysis.
  • Transcriptome analysis (RNA sequencing) to assess gene expression changes.
  • Genetic manipulation of KdmA levels in Aspergillus nidulans.

Main Results:

  • KdmA functions as an H3K36me3 demethylase.
  • KdmA influences 25-30% of the genome's transcriptome in submerged cultures.
  • KdmA deletion causes phenotypes under stress, including light sensitivity, and affects primary and secondary metabolism gene expression.

Conclusions:

  • KdmA is a crucial regulator of gene expression and epigenetic marks in Aspergillus nidulans.
  • KdmA plays a significant role in cellular adaptation to stress conditions.
  • Dysregulation of KdmA impacts fungal metabolism and viability, highlighting its importance in cellular homeostasis.

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