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Effect of desipramine on gene expression in the mouse frontal cortex - microarray study
Joanna Solich1, Magdalena Kolasa1, Maciej Kuśmider1
1Department of Pharmacology, Institute of Pharmacology Polish Academy of Sciences, Kraków, Poland.
Pharmacological Reports : PR
|February 26, 2015
Summary
Repeated desipramine (DMI) treatment altered gene expression in mouse frontal cortex. A week of DMI changed 18 genes linked to neuroplasticity, cytoskeletal changes, and cellular processes.
Area of Science:
- Neuroscience
- Molecular Biology
- Genomics
Background:
- Investigated gene expression changes in the mouse frontal cortex.
- Focused on repeated administration of desipramine (DMI) versus saline control.
Purpose of the Study:
- Identify genes differentially expressed in the frontal cortex following DMI treatment.
- Understand the molecular effects of DMI on the brain.
Main Methods:
- Utilized whole-genome microarray analysis.
- Examined gene expression profiles in mouse frontal cortex tissue.
Main Results:
- Identified 88 transcripts, representing 18 distinct genes, with altered expression.
- Found differential expression between DMI-treated and saline-treated mice.
- Highlighted key genes such as Spnb2, Mef2c, Ncam1, Hsp90ab1, Kif1b, Ddx6, and Gsk3b within a gene network.
Conclusions:
- One week of DMI administration significantly impacted gene expression.
- Affected genes are associated with crucial cellular functions including neuroplasticity, cytoskeletal regulation, calcium homeostasis, and translation.
- Suggests DMI's molecular mechanism involves alterations in these gene networks.
