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Expression of the ATP-gated P2X7 Receptor on M Cells and Its Modulating Role in the Mucosal Immune Environment

Sae-Hae Kim1, Ha-Yan Lee2, Yong-Suk Jang1

  • 1Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Korea. ; Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Korea.

Immune Network
|February 26, 2015
PubMed

Insights

This study identifies the ATP-gated P2X7 receptor (P2X7R) on M cells, revealing its role in regulating gastrointestinal mucosal immunity and immune responses to microbial molecules like ATP and LL-37.

Area of Science:

  • Gastrointestinal immunology
  • Cell biology
  • Microbial-epithelial interactions

Background:

  • Microbial interactions with gastrointestinal epithelial cells regulate mucosal immunity.
  • Microbe-derived molecules like ATP modulate mucosal immunity.
  • The role of the ATP-gated P2X7 receptor (P2X7R) in the gut, particularly on M cells in Peyer's patches, is poorly understood.

Purpose of the Study:

  • To characterize P2X7R expression on M cells.
  • To investigate the role of P2X7R in gastrointestinal mucosal immune regulation.
  • To determine P2X7R's function in immune enhancement mediated by ATP or LL-37.

Main Methods:

  • Immunohistochemistry to detect P2X7R expression on M cells.
  • In vitro assays to assess P2X7R function in response to ATP and LL-37.
  • Analysis of immune cell activation and cytokine production.

Main Results:

  • First report demonstrating P2X7R expression on M cells.
  • P2X7R activation by ATP or LL-37 enhances mucosal immune responses.
  • Characterization of P2X7R's contribution to purinergic signaling in the gut.

Conclusions:

  • P2X7R is expressed on M cells and plays a significant role in modulating gastrointestinal mucosal immunity.
  • P2X7R is a key mediator of immune responses to microbial products like ATP and LL-37.
  • Targeting P2X7R may offer therapeutic strategies for gut immune disorders.
Keywords:
AdjuvantImmune modulationM cellsMucosal immunityP2X7 receptor

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