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Updated: Apr 17, 2026
A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
Published on: May 10, 2013
Comparison of gamma camera-based methods to measure glomerular filtration rate in potential kidney donors
Shabana Saeed1, Smeera Saeed, Ambreen Khawar
1aDepartment of Medical Sciences, Pakistan Institute of Engineering and Applied Sciences, Islamabad bThe Kidney Centre, Rawalpindi, Pakistan.
Introduction:
The aim of this study was to compare two gamma camera-based methods (Gates' method and Inoue's method) for measuring glomerular filtration rate (GFR) with Russell's two plasma sample clearance method (2-PSC), which was taken as the gold standard.
Materials And Methods:
In 94 healthy potential kidney donors (25 women and 69 men), GFR was measured by means of the 2-PSC method and was compared with Gates' and Inoue's methods of assessing GFR after technetium-99m-labeled diethylene triamine penta-acetic acid (⁹⁹ mTc-DTPA) injection.
Results:
Inoue's method showed no statistically significant difference (P=0.22) with the gold standard (2-PSC); there was good correlation (r=0.69) and least bias (-1.91 ml/min/1.73 m², root mean squared error=11.85 ml/min/1.73 m²). Gates' method showed weak correlation (r=0.36) and tended to underestimate GFR by 21% with bias of 25 ml/min/1.73 m² and root mean squared error of 29.97.
Conclusion:
For a normal healthy adult population, Inoue's method gives a better measure of GFR in contrast to Gates' method when compared with 2-PSC method of GFR calculation.
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