Related Experiment Video
Updated: Apr 16, 2026
07:16
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
Published on: February 9, 2024
1.7K
Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme
Chaesun Hong1, Dong-Min Kim, Ahruem Baek
1Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea. kimde@konkuk.ac.kr.
Chemical Communications (Cambridge, England)
|February 26, 2015
Abstract:
We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
