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Related Experiment Videos

Intercellular adhesion molecule expression in the evolving human cutaneous delayed hypersensitivity reaction.

R E Lewis1, M Buchsbaum, D Whitaker

  • 1Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia.

The Journal of Investigative Dermatology
|November 1, 1989
PubMed
Summary
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Intercellular adhesion molecule-1 (ICAM-1) expression increases on skin cells during delayed hypersensitivity. This molecule is crucial for T-cell migration into the epidermis during immune responses.

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen (LFA-1).
  • ICAM-1 is expressed by antigen-presenting or target skin cells.
  • Its role in cutaneous delayed hypersensitivity is not fully elucidated.

Purpose of the Study:

  • To investigate the spatiotemporal expression of ICAM-1 during a delayed hypersensitivity response in human skin.
  • To correlate ICAM-1 expression with T-cell infiltration into the epidermis.

Main Methods:

  • Immunohistochemistry and immunoelectron microscopy on human skin.
  • Sequential evaluation over 96 hours after dinitrochlorobenzene challenge.
  • Assessment of mast cell degranulation and T-cell infiltrates.

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Main Results:

  • ICAM-1 expression was initially restricted to dermal microvasculature endothelium.
  • Progressive ICAM-1 staining observed in dermal dendritic cells and epidermal keratinocytes.
  • ICAM-1 expression by keratinocytes was directly associated with T-cell migration into the epidermis.
  • Mast cell degranulation and T-cell infiltrates were observed preceding significant epidermal ICAM-1 expression.

Conclusions:

  • ICAM-1 expression by epidermal cells is closely linked to T-cell migration in cutaneous delayed hypersensitivity.
  • ICAM-1 plays a critical role in facilitating T-cell entry into the epidermis during immune responses.
  • The findings provide insights into the cellular mechanisms of skin immune reactions.