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Related Concept Videos

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Related Experiment Video

Updated: Apr 16, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics.

Shinichi Hayashi1, Yasushi Okada2

  • 1Products Development Department 6, R&D Division, Olympus Corporation, 2951 Ishikawa-cho, Hachioji, Tokyo 192-8507, Japan.

Molecular Biology of the Cell
|February 27, 2015
PubMed
Summary

This study introduces a novel spinning disk superresolution microscope (SDSRM) that achieves high spatial resolution without sacrificing temporal resolution. This advancement enables detailed observation of live-cell dynamics, overcoming limitations of current superresolution techniques.

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Area of Science:

  • Microscopy
  • Biophysics
  • Cell Biology

Background:

  • Current superresolution (SR) microscopy techniques often compromise temporal resolution, limiting live-cell imaging.
  • Overcoming the diffraction limit is crucial for detailed cellular structure and dynamics visualization.

Purpose of the Study:

  • To develop a new superresolution fluorescence microscope with improved temporal resolution for live-cell imaging.
  • To adapt existing confocal microscopy platforms for enhanced superresolution capabilities.

Main Methods:

  • Developed a spinning disk superresolution microscope (SDSRM) based on confocal optics.
  • Utilized optical demodulation via a rotating disk's stripe pattern for SR signal recovery.
  • Modified a commercial spinning disk confocal microscope for SR imaging.

Main Results:

  • Achieved a spatial resolution of 120 nm, surpassing the diffraction limit.
  • Demonstrated significantly faster imaging (10x conventional SIM) with single-averaged images per SR frame.
  • Successfully visualized rapid dynamics of microtubules, mitochondria, lysosomes, and endosomes at 30-100 frames/s.

Conclusions:

  • The SDSRM offers a viable solution for high-resolution, high-speed live-cell imaging.
  • The method allows for straightforward upgrades of existing spinning disk confocal microscopes.
  • Enables detailed study of dynamic cellular processes previously unobservable with SR techniques.