The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration
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1The Pirbright Institute, High Street, Compton, Newbury, Berkshire, RG20 7NN, UK, ruth.hennion@pirbright.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2015
Abstract:
Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
