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Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Published on: April 28, 2008
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Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo
Joeri Kint1, Helena Jane Maier, Erik Jagt
1Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands, joeri.kint@wur.nl.
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2015
Abstract:
Quantification of the number of infectious viruses in a sample is a basic virological technique. In this chapter we provide a detailed description of three techniques to estimate the number of viable infectious avian coronaviruses in a sample. All three techniques are serial dilution assays, better known as titrations.
