Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poly-3-hydroxybutyrate production from used cooking olive oil by Cupriavidus necator DSM 545: Bioprocess development and OTR-k<sub>L</sub>a kinetic modelling in continuous fed-batch fermentation.

Bioresource technology·2026
Same author

Exploring phasin-polyhydroxyalkanoate interactions through in vivo and in vitro binding assays.

International journal of biological macromolecules·2026
Same author

Protocol for obtaining polyhydroxyalkanoates from microbial cultures: Production, quantification, and analytical assays.

STAR protocols·2025
Same author

Revealing the essential role of the lid in mclPHA intracellular depolymerase from Pseudomonas putida KT2440.

Applied microbiology and biotechnology·2025
Same author

Pushing the limits of bacterial cellulose for biomedicine: a review.

International journal of biological macromolecules·2025
Same author

Flexible feeding strategy for high-yield PHA bioprocessing in Cupriavidus necator H16 from anaerobically fermented industrial wastewater.

Bioresource technology·2025

Related Experiment Video

Updated: Apr 16, 2026

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.3K

Smart polyhydroxyalkanoate nanobeads by protein based functionalization.

Nina Dinjaski1, M Auxiliadora Prieto1

  • 1Polymer Biotechnology Lab, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

Nanomedicine : Nanotechnology, Biology, and Medicine
|February 28, 2015
PubMed
Summary

Researchers are developing bacterial polyhydroxyalkanoate (PHA) nanobeads for personalized medicine. These tunable biomaterials offer low-cost production and diverse biomedical applications, overcoming previous limitations.

Keywords:
DepolymeraseFunctionalized polyhydroxyalkanoatesGranule associated proteinsPhasinsSynthase

More Related Videos

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

2.1K
Procedure for Fabricating Biofunctional Nanofibers
09:39

Procedure for Fabricating Biofunctional Nanofibers

Published on: September 10, 2012

13.2K

Related Experiment Videos

Last Updated: Apr 16, 2026

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.3K
Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

2.1K
Procedure for Fabricating Biofunctional Nanofibers
09:39

Procedure for Fabricating Biofunctional Nanofibers

Published on: September 10, 2012

13.2K

Area of Science:

  • Nanobiotechnology
  • Biomaterials Science
  • Synthetic Biology

Background:

  • The 21st century is characterized by the rise of personalized medicine, increasing the demand for advanced biomaterials.
  • Bacterial polyhydroxyalkanoates (PHAs) are emerging as versatile biomaterials due to their biocompatibility and tunable properties.
  • Traditional methods for biomaterial production face challenges in cost and scalability.

Purpose of the Study:

  • To review the potential of bacterial polyhydroxyalkanoate (PHA) nanobeads as next-generation biomaterials.
  • To highlight the functionalization of PHA nanobeads using bacterial protein-binding systems for novel applications.
  • To discuss advancements in producing endotoxin-free PHA nanobeads for biomedical use.

Main Methods:

  • Harnessing bacterial carbon-storage granule production systems for PHA nanobead synthesis.
  • Utilizing proteins that bind PHA granules as tags for immobilizing recombinant proteins.
  • Implementing new platforms for producing endotoxin-free PHA nanobeads from Gram-positive bacteria.

Main Results:

  • Functionalized bacterial PHA nanobeads can be produced using straightforward, low-cost technologies.
  • PHA nanobeads can be engineered as functional nanocarriers for applications like bioseparation, enzyme immobilization, and diagnostics.
  • Development of endotoxin-free PHA nanobeads expands their potential in biomedical applications.

Conclusions:

  • Bacterial cell factories offer a sustainable alternative to chemical synthesis for biopolymer production.
  • PHA nanobeads represent a promising class of tunable biomaterials for innovative medicines and personalized biomedical approaches.
  • Further exploitation of bacterial systems can significantly expand the applications of biopolymers.