Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K
CRISPR01:59

CRISPR

60.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
60.3K
CRISPR01:59

CRISPR

19.0K
19.0K
Homologous Recombination02:31

Homologous Recombination

65.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

20.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
20.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The entropic view of aging: from thermodynamics to biology.

Life medicine·2026
Same author

Mechanisms and interventions of epigenetic aging.

Cell chemical biology·2026
Same author

Intervening in aging and related diseases with gene therapy techniques.

Cell reports. Medicine·2026
Same author

Single-nucleus interrogation of primate small intestinal aging reveals NCoR1 decline as a conserved feature that is reversed by metformin.

Nature aging·2026
Same author

Author Correction: CHIT1-positive microglia drive motor neuron ageing in the primate spinal cord.

Nature·2026
Same author

Mesenchymal drift: A convergent framework for the hallmarks of aging.

Cell·2026
Same journal

Correction to: Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation.

Protein & cell·2026
Same journal

mRNA-LNP delivery of individual longevity genes mitigates doxorubicin-induced progeroid phenotypes.

Protein & cell·2026
Same journal

Targeting the TBK1-p62 condensate axis restores sensitivity to EGFR-TKIs in resistant lung cancer.

Protein & cell·2026
Same journal

Recent progresses in cancer multidrug resistance and therapeutic options associated with protein damage response.

Protein & cell·2026
Same journal

LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability.

Protein & cell·2026
Same journal

3D chromatin dynamics in cellular neuroendocrine transformation.

Protein & cell·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.5K

CRISPR/Cas9 and TALE: beyond cut and paste.

Liping Deng1, Ruotong Ren1, Jun Wu2

  • 1National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.

Protein & Cell
|February 28, 2015
PubMed
Summary
This summary is machine-generated.

Nuclease-based genome editing tools like CRISPR/Cas and TALEs offer powerful applications. These technologies are advancing disease modeling, gene therapy, and gene expression regulation for genomic research.

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.5K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Nuclease-based genome editing is a rapidly advancing field with significant therapeutic potential.
  • CRISPR/Cas and Transcription Activator-Like Effector (TALE) nucleases are key technologies in this area.

Purpose of the Study:

  • To highlight the versatility of nuclease-based genome editing tools.
  • To explore their applications beyond gene editing, including gene expression regulation and genomic region analysis.

Main Methods:

  • Review of recent advances in CRISPR/Cas and TALE nuclease technologies.
  • Analysis of their functional mechanisms in genomic applications.

Main Results:

  • CRISPR/Cas and TALEs demonstrate efficacy as targeted gene expression regulators.
  • These nucleases can be employed to illuminate specific chromosomal structures and genomic regions.

Conclusions:

  • Nuclease-based genome editing tools possess broad applicability in biological research and medicine.
  • Their utility extends to gene therapy, disease modeling, and advanced genomic analysis.