Related Experiment Video
Updated: Apr 16, 2026

13:01
Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
138.0K
Anti-N antibody reacting at 37°C: An unusual occurrence interfering with routine testing: Two interesting cases
Vijay Kumawat1, Ashish Jain2, Neelam Marwaha2
1Department of Transfusion Medicine, Citizens Hospital, Nallagandla, Serilingampally, Hyderabad, India.
Asian Journal of Transfusion Science
|February 28, 2015
Abstract:
Most anti-N antibodies are naturally occurring, IgM antibodies, and not active above 25°C and are not clinically significant but IgG anti- N has also been described. Immune anti-N resulting from multiple transfusions does occur & has been implicated as the cause of hemolytic transfusion reactions and mild hemolytic disease of fetus and newborn. Anti- N reacting at room temperature can be a cause for ABO blood group discrepancy.

