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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
Published on: October 28, 2012
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A novel marker for undifferentiated human embryonic stem cells
Kiyoshi Higashi1, Masaki Yagi, Tatsuhiko Arakawa
11 Environmental Health Science Laboratory, Sumitomo Chemical Co. , Osaka, Japan .
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|February 28, 2015
Summary
Researchers identified CD147/basigin as a novel marker on human embryonic stem cells (hESCs). This molecule is expressed on undifferentiated hESCs and diminishes upon neural differentiation, suggesting its potential as a marker for pluripotency.
Area of Science:
- Stem Cell Biology
- Molecular Biology
- Cancer Research
Background:
- Human embryonic stem cells (hESCs) possess pluripotency, characterized by self-renewal and differentiation potential.
- Maintaining hESC pluripotency requires sustained expression of specific molecules and suppressed differentiation genes.
- Identifying novel markers for undifferentiated hESCs is crucial for stem cell research and therapeutic applications.
Purpose of the Study:
- To discover novel cell surface molecules expressed on undifferentiated human embryonic stem cells (hESCs).
- To investigate the expression pattern of a newly identified antigen during hESC differentiation.
- To identify the molecular identity of the novel antigen recognized by monoclonal antibody MAb2.
Main Methods:
- Generation of monoclonal antibodies against undifferentiated hESCs.
- Cell surface antigen characterization using Western blotting and immunoprecipitation.
- Proteomics analysis to identify the antigen recognized by MAb2.
- Immunohistochemical analysis of CD147/basigin expression in hESCs and lung cancer cells.
Main Results:
- Monoclonal antibody MAb2 recognized a cell surface antigen on undifferentiated hESCs.
- The MAb2 antigen expression decreased significantly during hESC differentiation into neural cells.
- Proteomics identified the MAb2 antigen as CD147/basigin, a protein also found on lung cancer cells.
- CD147/basigin protein expression was confirmed on the plasma membrane of undifferentiated hESCs.
Conclusions:
- CD147/basigin is expressed on the cell surface of undifferentiated human embryonic stem cells (hESCs).
- The expression of CD147/basigin diminishes upon neural differentiation, indicating its potential as a marker for pluripotency.
- CD147/basigin represents a novel marker for identifying undifferentiated hESCs.
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