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    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Signal Processing

    Background:

    • Scattering media, like biological tissues, impede deep imaging.
    • Acousto-optic imaging (AOI) offers a way to image through scattering media.
    • Achieving axial resolution in AOI has been a significant challenge.

    Purpose of the Study:

    • To introduce and demonstrate Fourier Transform acousto-optic imaging (FT-AOI) for enhanced resolution.
    • To evaluate the performance of FT-AOI in terms of signal-to-noise ratio and acquisition speed.
    • To assess the feasibility of FT-AOI for in vivo applications.

    Main Methods:

    • Implementation of an AOI system utilizing Fourier Transform (FT) analysis.
    • Employment of a CMOS smart-pixels sensor for real-time speckle pattern analysis.
    • Development of a measurement system for high-speed data acquisition.

    Main Results:

    • Successful demonstration of axial resolution in acousto-optic imaging.
    • Achieved signal-to-noise ratio comparable to AOI methods without axial resolution.
    • Demonstrated acquisition rates suitable for imaging dynamic biological samples.

    Conclusions:

    • FT-AOI is a viable technique for achieving axial resolution in scattering media.
    • The developed system offers a promising balance of resolution, signal quality, and speed.
    • FT-AOI holds potential for future biomedical imaging applications in living tissues.