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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

In vitro Mutagenesis

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Related Experiment Video

Updated: Apr 16, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

ENU mutagenesis in the mouse.

Rolf Stottmann¹, David Beier

¹Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.

Current Protocols in Mouse Biology

|February 28, 2015

Summary

This summary is machine-generated.

Researchers treated mice with N-ethyl-N-nitrosourea (ENU) to increase mutation rates. Mating schemes were then used to identify mice with observable changes linked to these genetic mutations.

Keywords:

alkylating agent genome sequencing per locus mutation frequency phenotype-driven mutagenesis spermatogonial stem cells

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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Area of Science:

Genetics and Genomics
Toxicology
Developmental Biology

Background:

Chemical mutagens are essential tools for genetic research.
N-ethyl-N-nitrosourea (ENU) is a potent mutagen used to induce random mutations in mammalian genomes.
Understanding mutation induction and detection is crucial for genetic studies.

Purpose of the Study:

To describe the methodology for inducing high mutation rates in mice using ENU.
To outline common breeding strategies for uncovering ENU-induced mutations.
To provide a framework for genetic research utilizing induced mutations.

Main Methods:

Laboratory mice were administered the mutagen N-ethyl-N-nitrosourea (ENU).
Genome-wide mutation rates were significantly elevated post-treatment.
Various mating schemes were employed to segregate and identify mutant phenotypes.

Main Results:

ENU treatment resulted in a substantial increase in mutation frequency across the mouse genome.
The described mating schemes effectively produced offspring exhibiting phenotypes associated with induced mutations.
This approach facilitates the study of gene function and the genetic basis of traits.

Conclusions:

ENU is a powerful tool for generating diverse mutations in mice.
Strategic mating is critical for the efficient identification of ENU-induced mutations.
This methodology supports forward genetic screens to investigate biological processes.