Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mutations01:35

Mutations

46.6K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
46.6K
Mutations01:39

Mutations

98.4K
Overview
98.4K
Biological Effects of Radiation02:59

Biological Effects of Radiation

19.9K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
19.9K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.9K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.9K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

42.8K
Overview
42.8K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

14.1K
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing Metal Ion Assignment Accuracy in Protein Data Bank Models via Elemental Spectroscopy.

Journal of chemical information and modeling·2026
Same author

Design, synthesis and evaluation of an uncharged broad spectrum quinoline-oxime hybrid for the reactivation of nerve agent-inhibited human acetylcholinesterase.

European journal of medicinal chemistry·2026
Same author

Decarboxylation via a Higher Electronic Excited State Drives LSSmOrange Photoconversion.

ACS physical chemistry Au·2026
Same author

Integrated structural dynamics uncover a new B<sub>12</sub> photoreceptor activation mode.

Nature·2026
Same author

Correction to "Mutations in Tau Protein Promote Aggregation by Favoring Extended Conformations".

JACS Au·2025
Same author

Perspective on a large-scale ligand structure characterization.

Acta crystallographica. Section D, Structural biology·2025
Same journal

Launching a new era for Short Communications in Journal of Synchrotron Radiation.

Journal of synchrotron radiation·2026
Same journal

Sagittal collimating diaboloid: a new grazing-incidence mirror surface for higher-throughput resonant inelastic X-ray scattering spectrometers.

Journal of synchrotron radiation·2026
Same journal

Synchrotron X-ray tomography and spectroscopy in numismatics: disclosing counterfeit practices in medieval silver coins.

Journal of synchrotron radiation·2026
Same journal

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026
Same journal

A robotic and high-throughput X-ray micro-computed tomography workflow.

Journal of synchrotron radiation·2026
Same journal

Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.

Journal of synchrotron radiation·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
11:24

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Published on: July 3, 2015

11.6K

Radiation damage to macromolecules: kill or cure?

Elspeth F Garman1, Martin Weik2

  • 1Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

Journal of Synchrotron Radiation
|February 28, 2015
PubMed
Summary
This summary is machine-generated.

Investigating X-ray radiation damage in macromolecular crystallography is crucial. New metrics and strategies are explored to identify and minimize damage, aiding structural biology research.

Keywords:
FELSAXSX-ray radiation damagemacromolecular crystallographyradiation damage induced phasingsimulations

More Related Videos

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
08:18

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

10.5K
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

12.1K

Related Experiment Videos

Last Updated: Apr 16, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
11:24

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

Published on: July 3, 2015

11.6K
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
08:18

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

10.5K
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

12.1K

Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • X-ray radiation damage is a significant challenge in macromolecular diffraction experiments.
  • Understanding and mitigating this damage is vital for accurate structural determination.

Purpose of the Study:

  • To present diverse, ongoing investigations into X-ray radiation damage in structural biology.
  • To explore novel methods for identifying, quantifying, and minimizing radiation damage.

Main Methods:

  • Development of a new metric using atomic B-factors to detect damaged residues.
  • Comparative studies on the radiation susceptibility of DNA and proteins.
  • Analysis of data from X-ray free-electron lasers (XFELs) to identify specific damage patterns.
  • Simulations of radiation damage influenced by XFEL temporal pulse profiles.
  • Investigation of radiation damage during scanning X-ray diffraction and small-angle X-ray scattering (SAXS) experiments.

Main Results:

  • A novel metric for identifying radiation-damaged amino acid residues has been proposed.
  • Differential susceptibility to radiation damage between protein and DNA components was observed.
  • Specific radiation damage signatures were detected in data from X-ray free-electron laser experiments.
  • Challenges in analyzing raw diffraction data from XFELs were highlighted.
  • Potential for using radiation damage-induced phasing for structure solution with XFELs was explored.
  • Simulations provided insights into radiation damage as a function of XFEL pulse profiles.
  • The influence of radiation damage on scanning X-ray diffraction measurements was quantified.
  • Strategies for minimizing radiation damage in SAXS experiments were considered.

Conclusions:

  • Ongoing research continues to advance the understanding of X-ray radiation damage in structural biology.
  • New methodologies and experimental strategies are being developed to address radiation damage.
  • Mitigating radiation damage is essential for improving the quality and reliability of structural data obtained from X-ray experiments.