Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stage- and Region-Dependent Proteomic Alterations in a Mouse Model of Creatine Transporter Deficiency.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Embryo transfer following IVF alters susceptibility to metabolic phenotypes in male mouse offspring compared to naturally conceived offspring.

Reproduction & fertility·2026
Same author

Why in vivo models of disease remain indispensable.

Disease models & mechanisms·2026
Same author

Hydroxytyrosol Mitigates Anxiety-Like Behaviors After a Traumatic Experience in Aged Mice in Parallel With Increased Neurogenesis in the Ventral and Dorsal Dentate Gyrus, and Preservation of Gut Microbiota Composition.

Journal of neurochemistry·2026
Same author

Improvement of Mouse Spermatozoa Freezing at -80°C With Ascorbic Acid 2-Glucoside at Thawing Phase.

Andrology·2026
Same author

Spiral ligament dysfunction and endocochlear potential loss drive hearing impairment in Niemann-Pick C1 mice.

Brain research bulletin·2026

Related Experiment Video

Updated: Apr 16, 2026

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

41.2K

Contemporary techniques for freezing mouse spermatozoa.

Mo Guan1, Debora Bogani, Susan Marschall

  • 1Mary Lyon Centre, Medical Research Council, Oxfordshire, United Kingdom.

Current Protocols in Mouse Biology
|February 28, 2015
PubMed
Summary
This summary is machine-generated.

Archiving mouse models is crucial for future research. Sperm freezing offers a cost-effective and efficient alternative to traditional embryo freezing for preserving valuable mouse genetic strains.

Keywords:
cryoprotectantepididymidesfreezingmousespermatozoa

More Related Videos

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
07:59

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

13.2K
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

32.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

41.2K
Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
07:59

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

13.2K
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
06:00

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Published on: November 18, 2011

32.6K

Area of Science:

  • Biomedical Research
  • Cryobiology
  • Genetics

Background:

  • Thousands of new mouse models are generated annually for biomedical research.
  • Maintaining mouse colonies is costly, making it uneconomical to store inactive strains.
  • Loss of genetic material occurs if retired mouse lines are not archived.

Purpose of the Study:

  • To describe efficient sperm freezing techniques for archiving mouse models.
  • To present alternatives to traditional, resource-intensive embryo cryopreservation.
  • To highlight the advantages of sperm banking for repositories.

Main Methods:

  • Utilizing refined cryoprotective agents for sperm preservation.
  • Employing improved in vitro fertilization techniques for viability assessment.
  • Describing two popular sperm freezing protocols using L-glutamine or monothioglycerol supplementation.

Main Results:

  • Sperm freezing circumvents the disadvantages of embryo freezing, such as high cost and invasive procedures.
  • Refined cryoprotective agents and in vitro fertilization techniques enable successful sperm cryopreservation.
  • Two specific sperm freezing techniques are detailed for repository use.

Conclusions:

  • Sperm freezing is a preferred method for archiving mouse strains due to its efficiency and lower cost.
  • This technique ensures the preservation of valuable genetic resources for future scientific endeavors.
  • The described methods provide practical solutions for mouse repositories to manage genetic diversity.