Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Domains01:18

Membrane Domains

8.4K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
8.4K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

4.5K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
4.5K
Cell Signaling in Plants01:25

Cell Signaling in Plants

7.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
7.1K
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

3.6K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.6K
Plasmodesmata02:32

Plasmodesmata

36.4K
The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
36.4K
Plasmodesmata01:20

Plasmodesmata

4.7K
In a multicellular organism, cells must communicate to work together in a coordinated manner. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>WUSCHEL-D1</i> upregulation enhances grain number by inducing formation of multiovary-producing florets in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Gibberellins promote polar auxin transport to regulate stem cell fate decisions in cambium.

Nature plants·2023
Same author

Loss of Multiple ABCB Auxin Transporters Recapitulates the Major <i>twisted dwarf 1</i> Phenotypes in <i>Arabidopsis thaliana</i>.

Frontiers in plant science·2022
Same author

Corrigendum to: Mutation of the membrane-associated M1 protease APM1 results in distinct embryonic and seedling developmental defects in Arabidopsis.

The Plant cell·2021
Same author

An ATP-Binding Cassette Transporter, ABCB19, Regulates Leaf Position and Morphology during Phototropin1-Mediated Blue Light Responses.

Plant physiology·2020
Same author

MultiSite Gateway-Compatible Cell Type-Specific Gene-Inducible System for Plants.

Plant physiology·2015
Same journal

Domain compositions of Arabidopsis Toll/Interleukin-1 Receptor/Resistance domain-containing TX14 proteins affect localization and induction of the hypersensitive response.

Journal of experimental botany·2026
Same journal

Shaping chloroplasts via galactolipids.

Journal of experimental botany·2026
Same journal

Hormonal crosstalk in the regulation of lignification: where do we stand?

Journal of experimental botany·2026
Same journal

Single-cell and spatial omics in plants: from cellular atlases to regulatory mechanisms.

Journal of experimental botany·2026
Same journal

Correction to: Beyond a plant hormone: ethylene receptors and signaling in microbes.

Journal of experimental botany·2026
Same journal

Single-Cell Atlases as Meta-Analytic Compasses for Developmental Biology: A Case Study Using the Arabidopsis Root.

Journal of experimental botany·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
11:44

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

14.9K

Membrane nanodomains in plants: capturing form, function, and movement.

Wiebke Tapken1, Angus S Murphy2

  • 1Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA.

Journal of Experimental Botany
|March 1, 2015
PubMed
Summary
This summary is machine-generated.

Plant plasma membrane nanodomains, rich in sterols and sphingolipids, organize proteins critical for cell communication and transport. These nanodomains show conserved functions across eukaryotes, with specific protein markers identified in plants.

Keywords:
Detergent-resistant membranesmembrane nanodomainsnanodomain functionordered membrane domainsplasma membrane ABC proteinssphingolipidssterols.

More Related Videos

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
05:54

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

2.8K
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.8K

Related Experiment Videos

Last Updated: Apr 16, 2026

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
11:44

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

Published on: September 28, 2013

14.9K
Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
05:54

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

2.8K
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.8K

Area of Science:

  • Cell Biology
  • Plant Biology
  • Biochemistry

Background:

  • The plasma membrane acts as a cellular barrier, with lipids scaffolding proteins vital for communication, signaling, immunity, and transport.
  • In eukaryotes, many plasma membrane proteins reside in specialized nanodomains enriched with sterols and sphingolipids.
  • Understanding these nanodomains is key to deciphering cellular processes.

Purpose of the Study:

  • To investigate the role and composition of plasma membrane nanodomains in plants.
  • To identify specific proteins and pathways associated with plant membrane nanodomains.
  • To compare plant nanodomain function with analogous structures in animals and fungi.

Main Methods:

  • High-resolution microscopy to visualize nanodomains.
  • Lipid dyes to probe membrane composition.
  • Pharmacological inhibitors and genetic mutants to study lipid biosynthesis.
  • Identification of nanodomain-associated proteins.

Main Results:

  • Plant membrane nanodomains are context-specific, similar to those in animals and fungi.
  • Flotillin and remorin proteins are conserved markers.
  • ATP binding cassette transporters (B and G subclasses) and fasciclin-like arabinogalactan proteins are identified as functional markers for plant nanodomains.
  • Pathways for nanodomain-associated protein trafficking were elucidated.

Conclusions:

  • Plant plasma membrane nanodomains are crucial for cellular functions and share conserved characteristics with other eukaryotes.
  • Specific proteins, including transporters and GPI-anchored proteins, play key roles in plant nanodomain organization and function.
  • These findings enhance our understanding of membrane compartmentalization and protein targeting in plants.