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Related Experiment Video

Updated: Apr 16, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Aptamer-based competitive electrochemical biosensor for brevetoxin-2.

Shimaa Eissa1, Mohamed Siaj2, Mohammed Zourob3

  • 1Institut National de la Recherche Scientifique, Centre - Énergie, Matériaux et Télécommunications, 1650, Boul. Lionel Boulet, Varennes, Que., Canada J3X 1S2; Dept. de Chimie et Biochimie, Université du Quebec à Montreal, Montreal, Que., Canada H3C 3P8.

Biosensors & Bioelectronics
|March 1, 2015
PubMed
Summary

Researchers developed a novel aptasensor for detecting brevetoxins (BTX), potent marine neurotoxins responsible for neurological shellfish poisoning (NSP). This biosensor offers a sensitive and reliable method for monitoring these harmful toxins in seafood.

Keywords:
AptasensorDNA aptamerElectrochemical Impedance spectroscopyIn vitro selection

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Area of Science:

  • Marine Biology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Brevetoxins (BTXs) are potent marine neurotoxins with increasing geographical distribution.
  • Neurological Shellfish Poisoning (NSP) is caused by BTX exposure.
  • Current analysis methods for marine toxins face ethical and technical challenges.

Purpose of the Study:

  • To develop an alternative, reliable detection method for BTX-2.
  • To create an electrochemical biosensor platform utilizing aptamers for BTX-2 detection.
  • To facilitate regulatory monitoring of marine toxins.

Main Methods:

  • In vitro selection was used to identify high-affinity DNA aptamers for BTX-2.
  • Fluorescence and electrochemical impedance spectroscopy (EIS) monitored aptamer-toxin binding.
  • A label-free competitive impedimetric biosensor was constructed using the selected aptamer (BT10).

Main Results:

  • The aptamer BT10 showed high affinity to BTX-2 (Kd = 42nM).
  • The impedimetric biosensor achieved a detection limit of 106pg/ml for BTX-2.
  • The aptasensor demonstrated high recovery rates in spiked shellfish extracts and specific cross-reactivity to BTX-2 and BTX-3.

Conclusions:

  • The developed aptasensor provides a sensitive and specific platform for BTX-2 detection.
  • This method can overcome limitations of current marine toxin analysis.
  • The aptasensor is suitable for routine detection of BTX-2 in food samples.