Related Experiment Video
Updated: Apr 16, 2026
06:47
A Murine Model of Vertical Sleeve Gastrectomy
Published on: December 18, 2017
12.0K
Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy
Yunmee Lho1, Carel W le Roux2, Hyeon Soo Park3
1Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea.
Obesity Surgery
|March 2, 2015
Summary
Vertical sleeve gastrectomy (VSG) in rats reduced food intake and body weight, while altering key metabolic pathways. VSG surgery suppresses hepatic glucose production, indicating significant metabolic shifts.
Area of Science:
- Metabolic research
- Surgical innovation
- Biochemistry
Background:
- Vertical sleeve gastrectomy (VSG) is a common bariatric surgery.
- Metabolic changes following VSG are not fully understood.
- This study investigates VSG's impact on liver and serum metabolism in a rat model.
Purpose of the Study:
- To evaluate metabolic alterations after VSG surgery.
- To identify key proteins and metabolites affected by VSG.
- To elucidate the mechanisms behind VSG-induced metabolic changes.
Main Methods:
- Proteomics and metabolomic profiling of liver and serum.
- VSG and sham surgeries in a rat model.
- Analysis of food intake, body weight, glucose, insulin, and thyroid hormones.
- Techniques included 2D electrophoresis, NMR, MS, immunofluorescence, and immunoblotting.
Main Results:
- VSG significantly decreased food intake and body weight.
- Blood glucose levels decreased, while insulin levels increased post-VSG.
- Key changes in hepatic gluconeogenic enzymes, metabolites (pyruvate, malate, lactate, NADH, NADPH), and energy status (AMP/ATP ratio) were observed.
- Thyroid hormones (T3 and fT4) decreased after VSG.
Conclusions:
- VSG suppresses hepatic glucose production.
- VSG induces significant metabolic reprogramming in the liver and serum.
- This study provides insights into the biochemical basis of VSG's metabolic effects.
Related Concept Videos
01:02Glucose Homeostasis: Regulation of Blood Glucose
5.5K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
5.5K
01:22Diabetes Mellitus: Type 2 and Gestational
5.9K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
5.9K
01:16Hormones Regulating Blood Glucose
9.1K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
9.1K
01:15Hypoglycemia and Glucagon
1.4K
Without prolonged fasting, healthy individuals maintain blood glucose levels above 3.5 mM due to a well-adapted neuroendocrine counterregulatory system that effectively prevents acute hypoglycemia, a potentially life-threatening condition. The primary clinical scenarios for hypoglycemia encompass diabetes treatment, inappropriate production of endogenous insulin or insulin-like substances by tumors, and the use of glucose-lowering agents in non-diabetic individuals. Notably, hypoglycemia in the...
1.4K
01:36Carbohydrate Metabolism
15.2K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
15.2K
01:18Metabolic States of the Body: The Postabsorptive State
1.7K
The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
1.7K