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Related Experiment Video

Updated: Apr 16, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Highly sensitive colorimetric sensor for Hg(2+) detection based on cationic polymer/DNA interaction.

Yingyue Zhu1, Yilin Cai2, Yibo Zhu1

  • 1School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China.

Biosensors & Bioelectronics
|March 3, 2015
PubMed
Summary
This summary is machine-generated.

This study presents a new colorimetric sensor for detecting mercury ions (Hg2+) using gold nanoparticles (AuNPs) and DNA. The sensor offers a simple, visual method for mercury detection in environmental samples.

Keywords:
AuNPs aggregationColorimetric sensorMercury ionT-Hg(2+)-T

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Area of Science:

  • Nanotechnology
  • Environmental Science
  • Analytical Chemistry

Background:

  • Accurate detection of ultralow mercury concentrations (Hg2+) remains a significant analytical challenge.
  • Existing methods may lack sensitivity, selectivity, or simplicity for widespread application.
  • Developing rapid and reliable mercury detection techniques is crucial for environmental monitoring and public health.

Purpose of the Study:

  • To develop a novel, highly sensitive, and selective colorimetric sensor for detecting mercury ions (Hg2+).
  • To utilize the aggregation of gold nanoparticles (AuNPs) triggered by a specific DNA-Hg2+ interaction for visual detection.
  • To establish a practical method for mercury detection applicable to real environmental samples.

Main Methods:

  • A three-component system involving gold nanoparticles (AuNPs), DNA, and a cationic polymer (PDDA).
  • Exploitation of thymine-Hg2+-thymine (T-Hg2+-T) induced hairpin turns in DNA to modulate AuNPs aggregation.
  • Colorimetric analysis based on the visual color change (wine-red to blue-purple) upon AuNPs aggregation.

Main Results:

  • A linear detection range of 0.25–500 nM for Hg2+ was achieved using absorption spectroscopy.
  • A low detection limit of approximately 0.15 nM for Hg2+ was determined under optimal conditions.
  • The sensor demonstrated high selectivity for Hg2+ over other common heavy metal ions and showed promising results in lake water analysis.

Conclusions:

  • The proposed colorimetric method provides a simple, naked-eye detectable, and sensitive approach for Hg2+ detection.
  • The DNA-AuNPs-PDDA system offers a robust platform for developing selective heavy metal sensors.
  • The method holds significant potential for practical environmental monitoring applications due to its performance and ease of use.