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Updated: Apr 16, 2026
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT
Published on: January 7, 2018
Glucose tolerance tests for systematic screening of glucose homeostasis in mice
Jan Rozman1,2,3, Birgit Rathkolb1,4,2,3, Susanne Neschen1,2,3
1Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany.
Abstract:
This article presents a detailed description of intraperitoneal and oral glucose tolerance tests in mice. The former is widely used in initial high-throughput phenotyping of mutant mice to assess a diabetic phenotype and alterations in glucose homeostasis. Each protocol provides a comprehensive description of each step in the workflow, including variation of the standard protocol under particular circumstances (e.g., sensitivity to food deprivation, excessive deviations in body composition, or need for extra blood samples for additional analyses). We also describe how reduction of body mass and body temperature can be used as additional readouts to monitor metabolic function in response to food deprivation.
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