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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Related Experiment Video

Updated: Apr 16, 2026

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

Basic techniques in mammalian cell tissue culture.

Katy Phelan¹, Kristin M May²

¹Hayward Genetics Program and Department of Pediatrics, Tulane University School of Medicine, New Orleans, Louisiana.

Current Protocols in Cell Biology

|March 3, 2015

Summary

This summary is machine-generated.

Learn essential mammalian cell culture skills to maintain cell structure and function. This guide covers aseptic techniques, media preparation, passaging, freezing, and viability assessment for reliable cell biology research.

Keywords:

aseptic technique freezing cells mammalian cells medium passaging cells tissue culture

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

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Last Updated: Apr 16, 2026

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

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Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Area of Science:

Cell Biology
Mammalian Cell Culture Techniques

Background:

Cultured mammalian cells are fundamental to numerous cell biology investigations.
Maintaining the integrity of cellular structure, function, and behavior in vitro is critical for reproducible results.

Purpose of the Study:

To outline the fundamental skills necessary for the successful maintenance and preservation of mammalian cell cultures.
To provide a practical guide for researchers new to cell culture methodologies.

Main Methods:

Adherence to strict aseptic techniques to prevent contamination.
Preparation of specialized cell culture media tailored to specific cell types.
Standard procedures for cell passaging, cryopreservation (freezing and storage), and recovery of frozen stocks.
Accurate enumeration of viable cells using established counting methods.

Main Results:

Successful preservation of cell structure, function, and behavior through proper technique.
Reliable recovery of cryopreserved cells with maintained viability.
Consistent cell populations for downstream experimental applications.

Conclusions:

Proficiency in basic cell culture techniques is essential for robust and reproducible cell biology research.
Standardized protocols for cell maintenance, cryopreservation, and viability assessment ensure the integrity of experimental models.