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Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples
A Brisson-Noël1, B Gicquel, D Lecossier
1Unité de Génie Microbiologique, Institut Pasteur, Paris.
Lancet (London, England)
|November 4, 1989
Abstract:
A method based on DNA amplification and hybridisation for the rapid detection of Mycobacterium tuberculosis was used to test 35 clinical specimens (sputum, gastric aspirate, abscess aspirate, biopsy sample) from 34 patients in whom tuberculosis was suspected. M tuberculosis was detected in 15 specimens, 2 of which were negative by standard microbiological criteria (microscopy and/or culture). 20 specimens, negative by standard methods, were also negative by the amplification method. M tuberculosis was also detected in peripheral blood samples of 2 of 4 patients with AIDS from whom the organism had been isolated.