Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.9K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.9K
CRISPR01:59

CRISPR

60.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
60.3K
CRISPR01:59

CRISPR

19.0K
19.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

20.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
20.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of Tcf7 regulators with clonally-resolved CRISPR screens identifies Trim28 as a mediator of CD8 T cell differentiation in tumors.

bioRxiv : the preprint server for biology·2026
Same author

CRISPR base editor screening identifies spectrum of MEN1 mutations impacting menin inhibitors in clinical trials.

Nature communications·2026
Same author

Genome-wide CRISPR interference screen identifies Clip2 as a novel regulator of osteocyte maturation and morphology.

The Journal of biological chemistry·2026
Same author

KDM6 Enzymes are the Mechanistic Targets of Mutant IDH that Dictate Replication Stress Sensitivity.

bioRxiv : the preprint server for biology·2026
Same author

A pooled CRISPR screen reveals genes critical for erythroblast enucleation.

bioRxiv : the preprint server for biology·2026
Same author

Sialylated CD43 forms a glyco-immune barrier that restrains antileukemic immunity.

Science (New York, N.Y.)·2026

Related Experiment Video

Updated: Apr 16, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.7K

Genetic screens and functional genomics using CRISPR/Cas9 technology.

Ella Hartenian1, John G Doench

  • 1Department of Molecular and Cellular Biology, University of California Berkeley, Berkeley, CA, USA.

The FEBS Journal
|March 3, 2015
PubMed
Summary

CRISPR/Cas9 gene editing technology offers a powerful new method for functional genomics research. This review covers CRISPR/Cas9 discovery, applications in genome-wide screens, and future potential for disease gene discovery.

Keywords:
CRISPRCas9RNAifunctional genomicsgene editinggenetic screenoff-target effectson-target activitypooled screenssgRNA

More Related Videos

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.5K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.7K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.5K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.6K

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Functional genomics aims to link gene dysfunction to disease by studying DNA, RNA, and protein.
  • CRISPR/Cas9 is a revolutionary gene editing tool enabling precise DNA modification.
  • Understanding gene function is crucial for disease research.

Purpose of the Study:

  • To review the discovery and characterization of CRISPR/Cas9 technology.
  • To compare CRISPR/Cas9 with existing genome engineering methods.
  • To discuss the application of CRISPR/Cas9 in genome-wide screens for gene discovery.

Main Methods:

  • Review of scientific literature on CRISPR/Cas9.
  • Comparison of CRISPR/Cas9 with other genome editing technologies (e.g., TALENs, ZFNs).
  • Analysis of CRISPR/Cas9 screening strategies and optimization techniques.

Main Results:

  • CRISPR/Cas9 offers high ease, speed, and accuracy in DNA editing.
  • CRISPR/Cas9 facilitates novel genome-wide genetic screens.
  • Optimization focuses on enhancing on-target activity and reducing off-target effects.

Conclusions:

  • CRISPR/Cas9 is a transformative technology for functional genomics and disease research.
  • Further research is needed to address challenges and explore future opportunities.
  • CRISPR/Cas9 is poised to accelerate the discovery of gene function and disease mechanisms.