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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Bioinformatic analysis of phage AB3, a phiKMV-like virus infecting Acinetobacter baumannii

J Zhang1, X Liu1, X-J Li2

  • 1Department of Geriatrics Medicine, The Third People's Hospital of Chongqing, Chongqing, China.

Genetics and Molecular Research : GMR
|March 3, 2015
PubMed

Insights

Acinetobacter baumannii phage AB3 was sequenced, revealing a double-stranded DNA genome. This characterization provides a foundation for phage therapy against multi-drug resistant bacteria.

Area of Science:

  • Microbiology and Virology
  • Genomics and Bioinformatics

Background:

  • Increasing multi-drug resistance in Acinetobacter baumannii necessitates novel therapeutic strategies.
  • Bacteriophages (phages) targeting A. baumannii are gaining attention for their potential in phage therapy.

Purpose of the Study:

  • To sequence the genome of Acinetobacter baumannii phage AB3.
  • To perform bioinformatic analysis of the phage AB3 genome.
  • To establish a foundation for genome remodeling and phage therapy applications.

Main Methods:

  • Isolation and sequencing of Acinetobacter baumannii phage AB3.
  • Bioinformatic annotation and analysis of the obtained genome sequence.
  • Genomic alignment and phylogenetic analysis using the RNA polymerase gene.

Main Results:

  • The phage AB3 genome is double-stranded DNA, 31,185 base pairs in length, with 97 open reading frames (>100 bp).
  • It contains 28 predicted genes, with 24 homologous to phage AB1; the coding sequence is on the negative strand (90.8%).
  • Genomic analysis classified phage AB3 as a phiKMV-like virus within the T7 phage family, similar to phage AB1.

Conclusions:

  • The complete genome sequence and bioinformatic analysis of phage AB3 are presented.
  • Phage AB3 exhibits characteristics of a phiKMV-like virus, related to phage AB1.
  • This study provides essential genomic data for future development of phage AB3 in therapeutic applications against A. baumannii.

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