Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

60.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
60.3K
CRISPR01:59

CRISPR

19.0K
19.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

20.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
20.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

eIF4G2-Dependent Translation Restrains Pancreatic Cancer Progression.

Cancer research·2026
Same author

Community Health Worker Feedback on an mHealth Intervention for Hypertension in Rural Guatemala: Mixed Methods Formative Study.

JMIR formative research·2026
Same author

eIF4G2-dependent translation restrains pancreatic cancer progression.

bioRxiv : the preprint server for biology·2026
Same author

Feasibility study of a novel mHealth clinical decision support application to enable Community Health Workers to manage hypertension in rural Guatemala.

medRxiv : the preprint server for health sciences·2026
Same author

High throughput identification of genetic regulators of microglial inflammatory processes in Alzheimer's disease.

Journal of neuroinflammation·2025
Same author

Neurofibromatosis Type 1 and the Search for Effective Tumor Therapies Using High-Throughput Drug Screening.

Current oncology (Toronto, Ont.)·2025
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.9K

Highly efficient Cas9-mediated transcriptional programming.

Alejandro Chavez1, Jonathan Scheiman2, Suhani Vora3

  • 11] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Nature Methods
|March 3, 2015
PubMed
Summary
This summary is machine-generated.

Researchers engineered a novel CRISPR-based transcriptional regulator, VP64-p65-Rta (VPR), for enhanced gene activation. This VPR system successfully activated multiple genes and promoted neuronal differentiation in human stem cells.

More Related Videos

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.7K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.5K

Related Experiment Videos

Last Updated: Apr 16, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.9K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.7K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.5K

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • CRISPR Technology

Background:

  • The Cas9 nuclease, guided by RNA, can be repurposed as a programmable transcription factor.
  • Current Cas9-based activators exhibit limited gene activation efficiency, restricting their therapeutic and research applications.

Purpose of the Study:

  • To develop an improved Cas9-based transcriptional regulator with enhanced gene activation capabilities.
  • To demonstrate the efficacy of the novel regulator in activating endogenous genes and directing cellular differentiation.

Main Methods:

  • Rational design and fusion of a tripartite activator, VP64-p65-Rta (VPR), to a nuclease-null Cas9.
  • Application of the VPR-Cas9 system for targeted activation of endogenous coding and noncoding genes.
  • Assessment of VPR-Cas9's ability to induce neuronal differentiation in human induced pluripotent stem cells (iPSCs).

Main Results:

  • The VPR fusion protein significantly enhanced gene activation compared to previous Cas9-based systems.
  • Simultaneous activation of multiple endogenous genes was achieved using the VPR-Cas9 regulator.
  • Successful stimulation of neuronal differentiation in human iPSCs was observed.

Conclusions:

  • The rationally designed VPR activator fused to nuclease-null Cas9 represents a powerful tool for robust gene activation.
  • This improved system holds promise for advancing gene therapy, synthetic biology, and stem cell research, particularly in neuronal differentiation applications.