Related Experiment Video
Updated: Apr 16, 2026
14:49
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Published on: October 27, 2011
39.8K
Simultaneous generation of many RNA-seq libraries in a single reaction
Alexander A Shishkin1, Georgia Giannoukos2, Alper Kucukural3
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Nature Methods
|March 3, 2015
Summary
RNAtag-Seq reduces RNA sequencing costs and time by creating multiple libraries in one reaction. This method provides comparable prokaryotic and eukaryotic data to traditional RNA-seq approaches.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Background:
- RNA sequencing (RNA-seq) is crucial for gene expression analysis.
- Traditional RNA-seq library preparation is time-consuming and expensive.
- Limited accessibility hinders broad application of RNA-seq.
Purpose of the Study:
- To introduce RNAtag-Seq, a novel method for generating multiple RNA-seq libraries simultaneously.
- To evaluate the efficiency and cost-effectiveness of RNAtag-Seq.
- To compare RNAtag-Seq data quality with traditional methods.
Main Methods:
- RNAtag-Seq enables the generation of multiple RNA-seq libraries within a single reaction.
- This approach streamlines the library construction process.
- Prokaryotic and eukaryotic samples were analyzed using RNAtag-Seq.
Main Results:
- RNAtag-Seq significantly reduces the time and cost per sample for RNA-seq library preparation.
- The method yields high-quality RNA-seq data.
- Data generated by RNAtag-Seq is comparable to traditional strand-specific RNA-seq approaches.
Conclusions:
- RNAtag-Seq offers a more accessible and efficient alternative for RNA sequencing.
- This method expands the utility of RNA-seq for diverse research applications.
- RNAtag-Seq provides reliable data for both prokaryotic and eukaryotic gene expression studies.
More Related Videos
Related Concept Videos
03:21RNA-seq
12.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.7K
03:00Next-generation Sequencing
102.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
102.1K
02:35RACE - Rapid Amplification of cDNA Ends
7.7K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.7K