Simultaneous generation of many RNA-seq libraries in a single reaction
Related Experiment Video
Updated: Apr 16, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Published on: October 27, 2011
Alexander A Shishkin1, Georgia Giannoukos2, Alper Kucukural3
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Abstract:
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
More Related Videos
Related Concept Videos
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
RACE - Rapid Amplification of cDNA Ends

